3P002 Enhancement of Protein Solubility by Adding Charged Amino Acids

Solubility of amino acids, sugars, and proteins*

The importance of biomolecules is well recognized nowadays, owing to their application in many industrial processes, particularly in the food, pharmaceutical, and cosmetic industries. Amino acids, carbohydrates, and proteins are the three types of biomolecules considered in this review. Solubilities of several amino acids and sugars have been measured in water and, more recently, in mixed solve...

Adding amino acids to the genetic repertoire.

Considerable progress has been made in expanding the number and nature of genetically encoded amino acids in Escherichia coli, yeast and mammalian cells in the past four years. To date, over 30 unnatural amino acids have been cotranslationally incorporated into proteins with high fidelity and efficiency by means of a unique codon and corresponding orthogonal tRNA-aminoacyl-tRNA synthetase pair....

Protein Solubility and Folding Enhancement by Interaction with RNA

While basic mechanisms of several major molecular chaperones are well understood, this machinery has been known to be involved in folding of only limited number of proteins inside the cells. Here, we report a chaperone type of protein folding facilitated by interaction with RNA. When an RNA-binding module is placed at the N-terminus of aggregation-prone target proteins, this module, upon bindin...

Effect of nitrogen fertilization on yield,protein and amino acids of irrigated mexipak wheat

The Role of the Positively Charged Amino Acids in Bacteriorhodopsin

This study has used chemical modification techniques to investigate the role of the only two types of positively charged amino acids, lysines and arginines, in the membrane-bound protein, bacteriorhodopsin (bR)• The results of these chemical modifications have significance both for the structure of bR and for the molecular mechanism of light-activated photocycling and proton pumping. The implic...